CV

Education

16. April 2013             Ph.D. from the Department of Systems Biology, Technical University of Denmark (DTU). Thesis title: “Structure, function and protein engineering in starch debranching enzyme systems.” Supervisors: Professor Birte Svensson and Associate professor Maher Abou Hachem (Enzyme and Protein Chemistry, Department of Systems Biology, DTU), and Senior Research Scientist Anette Henriksen (Novo Nordisk A/S).

August 2009               Master of Science in Engineering (Biotechnology), DTU, Lyngby.    Master Thesis (grade 12): “Biochemical properties and kinetic analysis of a glycoside hydrolase family 13_31 enzyme from the probiotic bacterium Lactobacillus acidophilus NCFM reveal broad specificity towards isomaltooligosaccharides and polymeric dextran.”

July 2007                    Bachelor Thesis (grade 11): “Production of recombinant barley limit dextrinase.”

June 2004                   Mathematical high school diploma (studentereksamen), Rønde Gymnasium, Denmark.

Employment

Nov. 2016-present          Assistant professor, Enzyme and Protein Chemistry group (EPC), DTU Bioengineering. Employed on a project financed by The Danish Council for Independent Research: “Roles and Structural Determinants of Low-affinity Carbohydrate-Protein Interactions”. Along with the research, I am following the Education in University Teaching at DTU.

Mar. 2014–Oct. 2016     Post-doctoral fellow at Lund University, Department of Biochemistry and Structural Biology in the group of Associate Professor Ingemar André and at DTU Bioengineering in the group of Professor Birte Svensson. The project was financed by an individual post doc grant from The Danish Council for Independent Research | Technology and Production (FTP) (see below). The title: “Design of proteinaceous inhibitors of industrially important starch degrading enzymes and proteases”.

Dec. 2012–Feb. 2014      Research assistant and post doctoral fellow (following the Ph.D. defence), Enzyme and Protein Chemistry group (EPC), Dept. of Systems Biology, DTU

Dec. 2009–Dec. 2012 Enrolled as a Ph.D. student at DTU (Dept. of Systems Biology)

Nov.–Dec. 2009          Research assistant, EPC, Dept. of Systems Biology, DTU

Feb.–May 2009           Assistant teacher in experimental biochemistry (approx. 6 hrs/week; Dept. of Systems Biology, DTU)

Grants/Awards

2010                            Travel grant (7000 DKK) from Otto Mønsteds Fond for conference participation in “Plant Polysaccharide and Applied Glycoscience Workshop” and “25th International Carbohydrate Symposium”, Tokyo, Japan.

2011                            Best poster prize at CoLuAa, conference for crystallographers from Copenhagen, Lund, and Aarhus.

2013                            Individual post doc stipend granted from The Danish Council for Independent Research – Technology and Production – covering 2 years of research – 2,439,577 DKK.

2013                            1st poster prize – “Outstanding poster”– at 5th Symposium on the Alpha-Amylase Family”, Slovakia

2013                            Awarded a Sapere Aude-Research Talent grant from the Danish Council for Independent Research – 481.104 DKK.

2015                            Travel grant (4450 DKK) from Otto Mønsteds Fond for conference participation in “11th Carbohydrate Bioengineering Meeting”, Helsinki, Finland.

Protein crystal structures deposited

Author on several protein crystal structures deposited in the Protein Databank (PDB): 4aio, 4cvw, 4j3s, 4j3t, 4j3u, 4j3v, 4j3w, and 4j3x.

Scientific focus areas

Protein chemistry, protein engineering, structure/function relationship of carbohydrate active enzymes, protein structure determination by X-ray crystallography, protein-protein interactions, protein design

Methods

Recombinant protein production in Escherichia coli and Pichia pastoris, enzymatic assays, surface plasmon resonance (SPR) analysis, analysis of carbohydrates by high-performance liquid chromatography (HPLC), X-ray crystallography, bioinformatics, computational protein design using ROSETTA, yeast surface display combined with Fluorescence-activated cell sorting (FACS)